High Density Spin Medium (1.1 g/ml)

¥ 648.00
所有价格不含税,不含运费。
用于制备不同密度的富集细胞、细菌和其他颗粒的工作溶液。
SKU
60-01103
Delivery between 2024年4月2日 and 2024年4月3日. Shipping from 德国.
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详细内容

高密度稀释培养基是一种即开即用的无菌培养基,用于制备不同密度的工作溶液,用于富集细胞、细菌和其他颗粒。工作溶液可通过高密度自旋培养基与高密度稀释培养基的稀释来制备。

应用

Preparation of Common Densities

To create your own density solution High Density Spin Medium and Deniuent Medium has to be mixed in appropriate quantities. You can use the table below for this calculation.
Common Density (g/ml) Volume (ml) Cell Types Volume of High Density Spin Medium (ml) Volume of Density Diluent Medium(ml)
35
65
1.069 100 Human Monocytes 15 85
1.077 100 Human PBMC 35 65
1.094 100 Human PMNC 77.5 22.5

Ensure that both solutions have the same temperature before mixing them. The High Density Spin Medium is highly viscous and needs to be pipetted very slowly. Alternativly it is possible to weigh out the releveant amounts of the media. The required mass will be calculated with the volume and the wanted density. Stir the mixture gently approximatly five minutes. Try to avoid air bubbles, otherwise you'll have to wait until the media is clear again before starting your experiment.

更多信息

更多信息
交付时间1-2
运输条件Room Temperature
储存条件室温,避光
规范性声明仅供研究使用。不用于诊断程序。
相位范围6.8 – 7.4

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