Leuko Spin Medium

¥ 264.00
所有价格不含税,不含运费。
用于体外分离人的新鲜外周血和水牛毛中的所有白细胞。
SKU
60-00091
Delivery between 2024年4月1日 and 2024年4月2日. Shipping from 德国.
quality - made in germany

  • ¥ 480.00 shipping to 中国 | Free shipping from ¥ 4,800.00
  • Shipping within 1-2 business days
  • DDU - Delivered Duty Unpaid
  • Secure Payment
  • Find all ordering information here.

详细内容

Leuko Spin是一种无菌的即用型密度培养基,用于从新鲜的人外周血或水皮层中分离所有白细胞,产量高。单核和多核(多叶核)细胞可通过Leuko Spin培养基的单步密度梯度离心来富集。通过与PBMC Spin双梯度离心相结合,可以在一个步骤中以高产率和高纯度分离粒细胞和PBMCs。富集的细胞可用于广泛的下游应用,如MACS(磁激活细胞分选,Miltenyi Biotec GmbH的商标)或干细胞研究。

应用

Separation scheme for Leukocyte enrichment with Leuko Spin Medium

At first carefully layer the sample material on top of the Leuko Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation step aspirate the upper layer (Plasma and dilution buffer). Then transfer the mononuclear and the polymorphonuclear cell layer to a new conical tube and wash the cells.


Density gradient centrifugation with Leuko Spin Medium.

Fig. 1: Isolation of all Leukocytes with Leuko Spin density separation medium.

After density gradient centrifugation with Leuko Spin Medium

Fig. 2: Enriched peripheral blood mononuclear and polymorphonuclear cells.

更多信息

更多信息
交付时间1-2
样品料Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
储存条件室温,避光
规范性声明仅供研究使用。不用于诊断程序。
相位范围6.8 – 7.4

著作