Cell Separation - Beads and Kits

Cell separation is the process to enrich a specific cell population such as CD3 positive Lymphocytes, CD14 positive Monocytes or CD19 positive B-Cell directly out of whole blood you have the option to use positive or negative (depletion) selection. Actually both isolation methods are antibody based. The antibodies recognize antigens (receptors) that the cells express on the surface. To support the enrichment the antibody can be coupled for example to other antibodies, fluorescent dyes or a solid phase.

pluriSelect offers products for positive isolation with the pluriBead system and negative isolation and depletion with pluriSpin system. Both technologies are without any magnetic beads

Positive Cell Separation
Cell isolation with pluriBead binds with specific antibody directly to the cells of interest. The cells will be "touched" by the target antibody. pluriBeads are detached after isolation.
Negative Cell Separation
pluriSpin method removes unwanted cells from your sample. The remaining cells of interest will be not touched. For example "B cell Enrichment" removes all cell types except B cells from the sample.
Depletion
pluriSpin depletion removes specific cell type from the sample material. For example "Monocyte Depletion" means Monocytes will be removed from the sample material.
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