Lympho Spin Medium / PBMC Spin Medium
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Lympho Spin Medium is a ready to use, sterile density gradient medium for the isolation of mononuclear cells (PBMC) in high yield from fresh peripheral whole blood and buffy coat by a simple centrifugation procedure. Mononuclear cells can be used for a wide range of downstream applications such as magnetic activated cell sorting (such as MACS1) or stem cell research.
Lympho Spin Medium can be used as substitute for Ficoll™,2 and Lymphoprep™,3 without any need to change the existing protocols.
Separation scheme for PBMC enrichment with PBMC Spin Medium
At first carefully layer the sample material on top of the PBMC Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.
Fig. 1: Preparation of PBMC with Lympho Spin Medium. The sample material will be overlayed on top of density gradient medium.
Fig. 2.: Layers after density gradient centrifugation. The PBMC can be found on top of the Lympho Spin Medium. Erythrocytes, Granulocytes and dead cells will pass the medium and can be found at the bottom of the tube.
1MACS is a trademark of Miltenyi Biotec GmbH; 2Ficoll™ is a trademark of GE Healthcare; 3Lymphoprep™ is a trademark of Axis-Shield / Alere
|Sample Material||Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution|
|Storage Condition||Room Temperature|
|Regulatory Statement||For research use only. Not for use in diagnostic procedures,|
Fig. 1: The picture of the flow cytometry analysis shows CD45+ peripheral blood mononuclear cells (PBMC) after density gradient centrifugation using PBMC Spin Medium from pluriSelect.
Fig.2: The composition of CD45+ PBMC after density gradient centrifugation with competitive density media using manufactor’s suggested protocol shows comparable results in fluorescence activated cell sorting analysis.
Fig. 3: The yield of PBMC using competitive density media shows no significant differences