Cell separation is the process to enrich a specific cell population such as CD3 positive Lymphocytes, CD14 positive Monocytes or CD19 positive B-Cell directly out of whole blood you have the option to use positive or negative (depletion) selection. Actually both isolation methods are antibody based. The antibodies recognize antigens (receptors) that the cells express on the surface. To support the enrichment the antibody can be coupled for example to other antibodies, fluorescent dyes or a solid phase.
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