High Density Spin Medium (1.1 g/ml)

€81.00
全ての価格は税抜き、送料抜きです。
細胞、細菌および他の粒子の濃縮のための異なる密度の作業溶液の調製のため。
SKU
60-01103
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詳細

密度希釈培地は、細胞、細菌、その他の粒子を濃縮するための異なる密度の作業溶液を調製するための、すぐに使用できる滅菌培地です。作業溶液は、高密度スピン培地を密度希釈培地で希釈して調製することができます。

アプリケーション

Preparation of Common Densities

To create your own density solution High Density Spin Medium and Deniuent Medium has to be mixed in appropriate quantities. You can use the table below for this calculation.
Common Density (g/ml) Volume (ml) Cell Types Volume of High Density Spin Medium (ml) Volume of Density Diluent Medium(ml)
35
65
1.069 100 Human Monocytes 15 85
1.077 100 Human PBMC 35 65
1.094 100 Human PMNC 77.5 22.5

Ensure that both solutions have the same temperature before mixing them. The High Density Spin Medium is highly viscous and needs to be pipetted very slowly. Alternativly it is possible to weigh out the releveant amounts of the media. The required mass will be calculated with the volume and the wanted density. Stir the mixture gently approximatly five minutes. Try to avoid air bubbles, otherwise you'll have to wait until the media is clear again before starting your experiment.

詳細情報

詳細情報
納期1-2
うんぱんじょうたいRoom Temperature
保管状態室温、光から保護
きせいぶん研究用としてのみ使用してください。診断手順での使用のためではありません。
フォーンレンジ6.8 – 7.4

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