1x RBC Lysis Buffer (250ml)

€48.00
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Buffer for the gentle lysis of red blood cells (RBC, erythrocytes)
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SKU
60-00050-12

この商品は他にもバリエーションがあります here. (100 ml, 500 ml)

Delivery between 2024/04/02 and 2024/04/03. Shipping from 日本.
quality - made in germany

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詳細

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. Nucleated RBCs are not effectively lysed with ammonium chloride. This 1x RBC Lysis Buffer is ready to use.

Components

  • NH 4 Cl (155 mMol/L)
  • KHCO 3 (10 mMol/L)
  • EDTA (0,1 mMol/L)

Protocol/h3>
  1. Ensure that the RBC Lysis Buffer is cold (4-8°C) .
  2. Dilute one volume of cell suspension with 10 volumes of 1× Red Blood Cell Lysis Solution.
  3. When working wih blood samples:
    Vortex for 5 seconds and incubate for 10-15 minutes (no more than 15 minutes) at 4-8°C.

    When working with tissue samples:
    Vortex for 5 seconds and incubate for 2 minutes at 4-8°C.
    Note: Observe turbidity to evaluate red blood cell lysis. Once the sample becomes clear red, lysis is complete.
  4. Vortex for 5 seconds and centrifuge at 300×g for 10 minutes at room temperature.
  5. Aspirate supernatant completely.
  6. Resuspend the cell pellet in an appropriate buffer and proceed to further applications.

詳細情報

詳細情報
大きさ250 ml
納期1-2
興味領域細胞生物学
不妊症無菌状態
フォーンレンジpH 7.4
うんぱんじょうたいRoom Temperature
保管状態4-8 °C
きせいぶんFor research use only. Not for use in diagnostic procedures.,

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