Leuko Spin Medium
Leuko Spin Medium is a ready to use, sterile density gradient medium for the isolation of Leukocytes in high yield from fresh peripheral blood and buffy coat by a simple centrifugation procedure.
Mononuclear and polymorphonuclear (multi-lobed nuclei) cells can be enriched by a simple density gradient centrifugation with Leuko Spin Medium. The enriched Leukocytes can be used for a wide range of downstream applications such as magnetic activated cell sorting (MACS1) or stem cell research.
1 MACS is a trademark of Miltenyi Biotec GmbH
Separation scheme for Leukocyte enrichment with Leuko Spin Medium
At first carefully layer the sample material on top of the Leuko Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation step aspirate the upper layer (Plasma and dilution buffer). Then transfer the mononuclear and the polymorphonuclear cell layer to a new conical tube and wash the cells.
Fig. 1: Isolation of all Leukocytes with Leuko Spin density separation medium.
Fig. 2: Enriched peripheral blood mononuclear and polymorphonuclear cells.
|Delivery Time (days)||1-2|
|Sample Material||Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution|
|Storage Condition||Room Temperature|
|Regulatory Statement||For research use only. Not for use in diagnostic procedures,|
|pH Range||6.8 – 7.4|
- Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function
Nina Doblinger, Andre Bredthauer, Morad Mohrez, Viola Hähnel, Bernhard Graf, Michael Gruber, Norbert Ahrens, 2019