Clinical Studies with pluriBead®
pluriBead® allows for positive non-magnetic cell isolation from any sample material. The procedure is simple: Sieve the pluriBead® with bound target cells down a strainer; pluriBead® with your target cells stays on top, while the unwanted cells run through. After detaching, target cells are ready.
pluriBead® and clinical studies
When performing clinical studies or trials, sample material from your patients is rare. The ability to separate more than one cell type from the same complex sample enables a new variety of analysis for studies in clinical trials and clinical research.
Current methods for blood analysis are serviceable for immediate stabilization of the blood (PAXGene®), but analysis of single cell populations (magnetic cell sorting) is time and manpower consuming. A lot of important sample material is wasted.
Their shortcomings are numerous, but pluriBead® helps to overcome these deficits.
|Limitation of possible targets (no proteins, viruses or microorganisms)||All types of targets, including proteins, viruses and microorganisms|
|Cost-intensive equipment, time-consuming procedure, high number of laboratory staff||No extra tools (except a mixer and a centrifuge), fast procedure, minimal staff requirement|
|PAXGene® and Tempus™ Blood RNA|
|Very fast, but only for global analysis||Simple cell isolation within 10 - 30 minutes|
|Cells cannot be sorted after stabilization||Flow-through can be used for further isolation steps|
|Small sample volume (2 ml whole blood per tube)||Works with samples from 0.2 - 45 ml|
|Magnetic cell separation|
|Only for one cell population, no separation of different cell types||Different targets from one sample via differently sized catcher particles and strainers|
|Sample preparation needed||Direct application in whole blood and other biological sera|
|Multiple steps (inclusive centrifugation) until stable RNA||Lyse cells directly on the pluriStrainer®
No centrifugation steps requiered