Bundle TwinSpin Tubes with Density Gradient Medium of Choise

$199.00
All prices excl. tax, excl. shipping.
TwinSpin centrifuge tube to support density gradient centrifugation.
SKU
45-90006-10
Delivery between Mar 20, 2024 and Mar 21, 2024. Shipping from United States.

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Details

TwinSpin centrifugation tubes can be filled with Density Gradient Medium (DGM) for an optimal separation of cells from whole blood and bone marrow. The TwinSpin is comprised of a standard 15 ml and an inner tube. The inner tube has an open bottom which is submersed in the DGM. Diluted anti-coagulated blood or bone marrow can simply be pipetted into the TwinSpin. The sample lays on top of the DGM inside the inner tube. During centrifugation, Leukocytes, lymphocytes and PBMCs are separated from unwanted erythrocytes and granulocytes, depending on the DGM. The result is an inter-phase enriched with target cells above the DGM.

The erythrocytes will sediment through the density gradient media out of the inner tube at the bottom of the outer tube. When separation is complete, just remove the inner tube. The elastic cap functions as valve. By pushing the cap down, the collection tube becomes a pipette. The contents can be collected drop by drop.

Bundle TwinSpin tubes with DGM of your choice.

DGM explanation

Leuko Spin - For the isolation of leukocytes from whole blood or bone marrow
Lympho Spin - For the isolation of PBMC from whole blood or bone marrow
Lympho Spin 24+ - For the isolation of PBMC from whole blood older than 12 hours or bone marrow
PLT Spin - For the isolation of platelets from whole blood or bone marrow

Application

Protocol for the use of TwinSpin® in combination with density gradient media.

twinspin scheme - step 1
Remove transport stopper
twinspin scheme - step 1
Fill in sample material
twinspin scheme - step 1
Spin
twinspin scheme - step 1
Collect
icon - density gradient media Density Gradient Media
icon - plasma Plasma
icon - red blood cells Red blood cells and unwanted cells
icon - desired cells Desired cells

Find full protocol in downloads section

More Information

More Information
PrefilledUnfilled
Delivery Time (days)1-2
Storage ConditionRoom Temperature. Keep dark, do not use when brownish coloration
Shipping ConditionRoom Temperature
Regulatory StatementFor research use only. Not for use in diagnostic procedures.