1x RBC Lysis Buffer

All prices excl. tax, excl. shipping.
Buffer for the gentle lysis of red blood cells (RBC, erythrocytes)
Delivery between Jun 19, 2024 and Jun 20, 2024. Shipping from United States.
quality - made in germany

Order directly
Pay after receipt
No account required
  • $40.00 shipping to United States
  • Shipping within 1-2 business days
  • Secure Payment
  • DAP - Delivered at Place
  • Find all ordering information here


Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. Nucleated RBCs are not effectively lysed with ammonium chloride. This 1x RBC Lysis Buffer is ready to use.


  • NH4 Cl (155 mMol/L)
  • KHCO3 (10 mMol/L)
  • EDTA (0.1 mMol/L)


  1. Ensure that the RBC Lysis Buffer is cold (4-8°C) .
  2. Dilute one volume of cell suspension with 10 volumes of 1× Red Blood Cell Lysis Solution.
  3. When working wih blood samples:
    Vortex for 5 seconds and incubate for 10-15 minutes (no more than 15 minutes) at 4-8°C.

    When working with tissue samples:
    Vortex for 5 seconds and incubate for 2 minutes at 4-8°C.
    Note: Observe turbidity to evaluate red blood cell lysis. Once the sample becomes clear red, lysis is complete.
  4. Vortex for 5 seconds and centrifuge at 300×g for 10 minutes at room temperature.
  5. Aspirate supernatant completely.
  6. Resuspend the cell pellet in an appropriate buffer and proceed to further applications.

More Information

More Information
Delivery Time (days)1-2
Area of interestCell Biology
pH RangepH 7.4
Shipping ConditionRoom Temperature
Storage Condition4-8 °C
Regulatory StatementFor research use only. Not for use in diagnostic procedures.,

Downloads 0