Density Diluent Medium
High Density Spin Medium is a ready to use, sterile medium for the preparation of different density working solutions for the enrichment of cells, bacteria and other particles. The working solutions can be prepared by the dilution of High Density Spin Medium with our Density Diluent Medium.
Preparation of Common DensitiesTo create your own density solution High Density Spin Medium and Deniuent Medium has to be mixed in appropriate quantities. You can use the table below for this calculation.
|Common Density (g/ml)||Volume (ml)||Cell Types||Volume of High Density Spin Medium (ml)||Volume of Density Diluent Medium(ml)|
Ensure that both solutions have the same temperature before mixing them. The High Density Spin Medium is highly viscous and needs to be pipetted very slowly. Alternativly it is possible to weigh out the releveant amounts of the media. The required mass will be calculated with the volume and the wanted density. Stir the mixture gently approximatly five minutes. Try to avoid air bubbles, otherwise you'll have to wait until the media is clear again before starting your experiment.
|Delivery Time (days)||1-2|
|Shipping Condition||Room Temperature|
|Storage Condition||Room temperature, protect from light|
|Regulatory Statement||For research use only. Not for use in diagnostic procedures.|
|pH Range||6.8 - 7.4|