Monocytes Spin Medium
Monocytes Spin Medium is a ready-to-use, sterile density medium for the enrichment of monocytes in a high yield from fresh peripheral blood or buffy coat through single step density centrifugation. Depending on the sample a purity of 60-80% is possible. Monocytes Spin is a gentle alternative to bead-mediated monocyte enrichments, especially for downstream applications like cell culture and differentiation.
|Delivery Time (days)||1-2|
|Sample Material||Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution|
|Storage Condition||Room temperature, protect from light|
|Regulatory Statement||For research use only. Not for use in diagnostic procedures.|
|pH Range||6.8 – 7.4|
Fig 1.: Enriched cells with Monocyte Spin Medium.
FSC/SSC plot of CD45+ cells after enrichment through density gradient centrifugation with Monocyte Spin Medium.
Fig 2.: Monocytes in CD45+ population.
huCD14+ plot gated on huCD45+ shows enrichment of monocytes up to 70% after density gradient centrifugation with Monocyte Spin Medium.
Fig 3.: Enriched cells with Lympho Spin Medium.
FSC/SSC Plot of CD45+ cells after enrichment through density gradient centrifugation with PBMC Spin Medium. typically PBMC consist of 70-90% lymphocytes and 10-25% monocytes. In older blood contamination with granulocytes are possible.
Fig 4.: Monocytes in CD45+ population.
huCD14+ plot gated on huCD45+ cells shows that 24,5% of the PBMC Spin enriched cells are monocytes. This proportion depends on the individual.