High Density Spin Medium (1.1 g/ml)

$70.00
All prices excl. tax, excl. shipping.
For the preparation of different density working solutions for the enrichment of cells, bacteria and other particles.
SKU
60-01103
Order within 03min for same day dispatch. Delivery between 1/17/21 and 1/18/21. Shipping from United States.
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Details

Density Diluent Medium is a ready to use, sterile medium for the preparation of different density working solutions for the enrichment of cells, bacteria and other particles. The working solutions can be prepared by the dilution of High Density Spin Medium with Density Diluent Medium.

Application

Preparation of Common Densities

To create your own density solution High Density Spin Medium and Deniuent Medium has to be mixed in appropriate quantities. You can use the table below for this calculation.
Common Density (g/ml) Volume (ml) Cell Types Volume of High Density Spin Medium (ml) Volume of Density Diluent Medium(ml)
35
65
1.069 100 Human Monocytes 15 85
1.077 100 Human PBMC 35 65
1.094 100 Human PMNC 77.5 22.5

Ensure that both solutions have the same temperature before mixing them. The High Density Spin Medium is highly viscous and needs to be pipetted very slowly. Alternativly it is possible to weigh out the releveant amounts of the media. The required mass will be calculated with the volume and the wanted density. Stir the mixture gently approximatly five minutes. Try to avoid air bubbles, otherwise you'll have to wait until the media is clear again before starting your experiment.

More Information

More Information
Delivery Time (days)1-2
Shipping ConditionRoom Temperature
Storage ConditionRoom temperature, protect from light
Regulatory StatementFor research use only. Not for use in diagnostic procedures.
pH Range6.8 – 7.4

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