Lympho Spin Medium / PBMC Spin Medium
PBMC Spin is a sterile, ready-to-use density medium for the isolation of peripheral blood mononuclear cells from fresh human blood or buffy coat with a high yield. Cells can be enriched by a single-step density gradient centrifugation with PBMC Spin Medium. Through combination with Leuko Spin Medium in a double gradient centridugation it is possible to isolate and separate granulocytes and PBMCs in one step with high yield and purity. PBMC Spin Medium can be used as substitute for Ficoll-Paque1, Pancoll2 and Lymphoprep without any need to change the existing protocols. The enriched cells can be used for a wide range of downstream applications such as MACS, pluriBead or stem cell research.
1 Ficoll-Paque is a trademark of GE Healthcare/Cytiva
2 Pancoll is a trademark of PAN-Biotech GmbH
3 Lymphoprep is a trademark of Alere Technologies Inc
4 MACS is a trademark of Miltenyi Biotec GmbH
Separation scheme for PBMC enrichment with PBMC Spin Medium
At first carefully layer the sample material on top of the PBMC Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.
Fig. 1: Preparation of PBMC with Lympho Spin Medium. The sample material will be overlayed on top of density gradient medium.
Fig. 2.: Layers after density gradient centrifugation. The PBMC can be found on top of the Lympho Spin Medium. Erythrocytes, Granulocytes and dead cells will pass the medium and can be found at the bottom of the tube.
|Delivery Time (days)||1-2|
|Sample Material||Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution|
|Storage Condition||Room temperature, protect from light|
|Regulatory Statement||For research use only. Not for use in diagnostic procedures.|
|pH Range||6.8 – 7.4|
Fig. 1: The picture of the flow cytometry analysis shows CD45+ peripheral blood mononuclear cells (PBMC) after density gradient centrifugation using PBMC Spin Medium from pluriSelect.
Fig.2: The composition of CD45+ PBMC after density gradient centrifugation with competitive density media using manufactor’s suggested protocol shows comparable results in fluorescence activated cell sorting analysis.
Fig. 3: The yield of PBMC using competitive density media shows no significant differences