Monocytes Spin Medium, 250 ml

$65.00
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For in vitro enrichment of monocytes from fresh human peripheral blood and buffy coat.
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SKU
60-00095-11

This product is available in other variations here. (100 ml, 500 ml)

Delivery between 11/2/20 and 11/3/20. Shipping from United States.
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Details

Monocyte Spin Medium is a ready to use, sterile medium for enrichment (isolation) of monocytes in high yield from fresh peripheral blood and buffy coat. The monocytes can be enriched by a simple density gradient centrifugation with monocyte Spin Medium up to a purity off 50-60%.

More Information

More Information
Size250 ml
Delivery Time (days)1-2
Storage ConditionRoom Temperature
Regulatory StatementFor research use only. Not for use in diagnostic procedures,
pH Range6.8 – 7.4

Data

Enrichment with Monocytes Spin Medium
Monocytes with regular PBMC medium
Enriched cells with Monocyte Spin Medium
Monocytes in CD45 population
Enriched cells with Lympho Spin Medium
Monocytes in CD45 population

Fig 1.: Enriched cells with Monocyte Spin Medium.

FSC/SSC plot of CD45+ cells after enrichment through density gradient centrifugation with Monocyte Spin Medium.

Fig 2.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ shows enrichment of monocytes up to 70% after density gradient centrifugation with Monocyte Spin Medium.

Fig 3.: Enriched cells with Lympho Spin Medium.

FSC/SSC Plot of CD45+ cells after enrichment through density gradient centrifugation with PBMC Spin Medium. typically PBMC consist of 70-90% lymphocytes and 10-25% monocytes. In older blood contamination with granulocytes are possible.

Fig 4.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ cells shows that 24,5% of the PBMC Spin enriched cells are monocytes. This proportion depends on the individual.

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