Monocytes Spin Medium, 250 ml

$65.00
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For in vitro enrichment of monocytes from fresh human peripheral blood and buffy coat.
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SKU
60-00095-11

This product is available in other variations here. (100 ml, 500 ml)

Delivery between 9/27/21 and 9/28/21. Shipping from United States.
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Details

Monocytes Spin Medium is a ready-to-use, sterile density medium for the enrichment of monocytes in a high yield from fresh peripheral blood or buffy coat through single step density centrifugation. Depending on the sample a purity of 60-80% is possible. Monocytes Spin is a gentle alternative to bead-mediated monocyte enrichments, especially for downstream applications like cell culture and differentiation.

More Information

More Information
Size250 ml
Delivery Time (days)1-2
Storage ConditionRoom temperature, protect from light
Regulatory StatementFor research use only. Not for use in diagnostic procedures.
pH Range6.8 – 7.4

Data

Enrichment with Monocytes Spin Medium
Monocytes with regular PBMC medium
Enriched cells with Monocyte Spin Medium
Monocytes in CD45 population
Enriched cells with Lympho Spin Medium
Monocytes in CD45 population

Fig 1.: Enriched cells with Monocyte Spin Medium.

FSC/SSC plot of CD45+ cells after enrichment through density gradient centrifugation with Monocyte Spin Medium.

Fig 2.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ shows enrichment of monocytes up to 70% after density gradient centrifugation with Monocyte Spin Medium.

Fig 3.: Enriched cells with Lympho Spin Medium.

FSC/SSC Plot of CD45+ cells after enrichment through density gradient centrifugation with PBMC Spin Medium. typically PBMC consist of 70-90% lymphocytes and 10-25% monocytes. In older blood contamination with granulocytes are possible.

Fig 4.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ cells shows that 24,5% of the PBMC Spin enriched cells are monocytes. This proportion depends on the individual.

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